Description:
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Carnation event 26407 was developed by Agrobacterium tumefaciens-mediated transformation of conventional carnation Cerise Westpearl to express anthocyanins in the petals conferring a mauve colour to the flowers.
The transformation vector pCGP2355 contained the following expression cassettes:
- the cytochrome b5 (cytb5) cassette,
- the flavonoid 3′,5′-hydroxylase (f3′5′h) cassette,
- the acetolactate synthase cassette (als) conferring tolerance to sulfonylurea herbicides.
Expression of cytb5 and f3′5′h, together with endogenous genes in the anthocyanin biosynthesis pathway, give rise to the anthocyanins delphinidin and cyanidin in the petals.
26407 contains one insert consisting of the transfer DNA (T-DNA) region of the transformation vector pCGP2355. Southern blot and PCR analyses indicated that no plasmid backbone sequences had been integrated.
. The stability of the newly introduced trait (mauve flower colour) was observed over multiple generations.
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Links regarding to this GMO:
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BCH http://bch.cbd.int/database/record.shtml?documentid=104609,
ISAAA http://www.isaaa.org/gmapprovaldatabase/event/default.asp?EventID=361
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Transformation / Modification technique:
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Agrobacterium-induced
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